What is substitution splice?

Substitution splice is a technique that involves replacing a particular segment of genetic material with another segment from another source. This technique is commonly used in genetic engineering to modify or alter the DNA of a given organism. Genes containing desirable traits are isolated from one organism and then spliced into another organism's DNA to produce a new modified organism with the desired trait. Substitution splicing requires specialized enzymes called restriction endonucleases, which can cut DNA at specific points, and ligases that can join the cut ends to form new fragments. This technique is widely used in agriculture, medicine, and biotechnology to produce genetically modified crops, create new vaccines, and treat genetic disorders. However, it also raises ethical concerns regarding the safety and sustainability of genetically modified organisms.